Quant-iT PicoGreen dsDNA Assay Kit (1mL) Quant-iT PicoGreen dsDNA Reagent (1mL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P7589 P7581: Quant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen. The Quant-iT PicoGreen dsDNA Assay Kit from Thermo Fisher Scientific is more specific for DNA and is about 1000 times more sensitive than traditional absorbance methods. Average 86 stars, based on 1. low-range. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Quantitation using Quant-iT Assay Kits. Selectively detect dsDNA from a wide variety of sources, and even in presence of. RCA products may be directly quantified using the Quant-iT® PicoGreen® dsDNA Assay Kit or with a Qubit® Fluorometer after dilution to the appropriate detection range. 0 fluorometer—provides state-of-the-art reagents for sensitive and selective quantitation of DNA, RNA or protein samples using a standard fluorescence microplate reader or the Qubit 2. Application observe demonstrating so with Molecular Devices SpectraMax® microplate book and the Quant-iT PicoGreen assay, users can reliably size concentrations as low as 50 pg/mL of double-stranded DNA. The DNA amount in each library was quantified with the Quant-iT PicoGreen dsDNA Assay Kit and with the Agilent 2200 TapeStation. 10 x 100 µL. The PicoGreen dsDNA quantitation reagent and kits are ideal for PCR-based assays, microarray samples, DNA damage assays, enzyme activity assays, genomic DNA. PicoGreen dsDNA quantitation reagents are. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Simply dilute the reagent 1:200, load 200 µL into the wells of a microplate, add 1–20 µL sample, mix, then read the. • Specific for dsDNA in the presence of RNA. QuantiFluor® ONE dye sensitivity range is 0. Vortex solution briefly to mix well and centrifuge for 5 sec to bring liquid to bottom of tube; then dispense for use in the assay. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using the PicoGreen dsDNA quantitation assay and reagent. Quant-iT™ PicoGreen ® dsDNA Reagent (10 vials, each containing 100 µL aliquots) 25 mL of 20X TE buffer; 1 mL of 100 µg/mL Lambda DNA (dsDNA standard) These jellies cheapest cialis uk and pills are can be ingested with or without food. 反应次数. Triplicate 10 μL samples of λ DNA (Ο), E. The Quant-iT™ OliGreen™ ssDNA Assay Reagent enables researchers to quantify as little as 1 ng/mL oligonucleotide or ssDNA (200 pg in a 200 µL assay volume) with a. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. As little as 2. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. Quantitation Range. 특징: Quanti-iT Picogreen을 이용하면 낮은 농도의 DNA 측정에 유리. P11495. Abstract. Quantitation Range. The high standard corresponds to the same concentration as the highest concentration dsDNA standard in the Quant-iT Assay Kits and can be diluted into the standard series. Quant-iT OliGreen ssDNA Assay Kits contain an ultrasensitive, green-fluorescent nucleic acid stain for quantitating oligonucleotides and single-stranded DNA (ssDNA) in solution. Picogreen assays are compatible with most microplate readers and fluorometers. Buy from Supplier. Quant-iT PicoGreen dsDNA Assay Kit. The kits provide concentrated assay reagent, dilution buffer, and prediluted DNA standards. Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile, distilled, DNase-free water. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. For duplicate-well droplet. Quant-iT PicoGreen dsDNA Assay Kit. The assay was prepared for 184 aliquots of DNA sample and five DNA standards, including one blank. (dsDNA) High Sensitivity (HS) assay, Quant-iT PicoGreen Assay, Bioanalyzer HS DNA assay, and NanoDrop Onec. Quantify nucleic acids and proteins using four user-selectable fluorescent. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Determine the DNA concentration on the basis of absorbance at 260 nm (A 260) in a cuvette with a 1 cm pathlength; an A 260 of 0. • Suitable for use in 96- and 384-well plate formats. Library quality and average fragment size was assessed using a Bioanalyzer (Agilent, G2939A) High Sensitivity DNA chip (Agilent, 5067-4626). Katalognummer: P11496. The limit of detection for the 20/20n is less than 45 pg in 100 µL total volume. BR reagent (Component A) 1. 8. The assay is highly selective for double- The assay is highly selective for double- stranded DNA over RNA (see “Assay selectivity” on page 1), and in the range of 0. The Quant-iT™ PicoGreen® DNA assay is the oldest assay and the most general-purpose. 5 ng/ml of dsDNA were detected with minimal effect in the presence of ssDNA, RNA, and free nucleotides, which is comparable with InvitrogenTM Quant-iT™ PicoGreen® dsDNA Reagent. The Quant-iT dsDNA High-Sensitivity Assay Kit and the Quant-iT dsDNA Broad-Range Assay Kit make DNA quantification easy and accurate. Quant-iT ™ PicoGreen ™ dsDNA Assay Kit 1 mL assay kitQuant-iT™ PicoGreen® dsDNA Reagent and Kits | 4 Experimental Protoco l DNA Standard Curve 1. 95 mL TE for the . 500/525. Thermo Fisher Scientific公司的Quant-iT PicoGreen dsDNA 检测试剂盒对DNA更特异，比传统光吸收方法的灵敏度高约 1000倍。 如产品手册所述，此实验在微孔板格式中采用单 一染料浓度的动态范围从250 pg/mL到1000 ng/mL。在这 里，我们证明了使用Molecular Devices公司SpectraMax®Quant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen ssDNA Reagent: ssDNA : 200 pg–2 µg : 500/525 : 2,000 : O11492 O7582: Quant-iT RiboGreen RNA Assay Kit Quant-iT. Picogreen assays are compatible with most microplate readers and fluorometers. 21 DiB) [22]. Q33130). The Quant-iT 1X dsDNA HS Assay Kit is accurate for initial sample concentrations ranging from 10 pg/µL to 100 ng/µL, providing a core detection range of 0. The Quant-iT™ dsDNA Broad-Range Assay Kit makes DNA quantitation easy and accurate. 2022. The The lambda DNA standard, provided at 100 µg/mL in the Quant-iT ™ PicoGreen dsDNA Assay Kit, is diluted 50‑fold in TE to make the 2 µg/mL working solution. Quant-iT dsDNA BR buffer (Component B) 250 ml c. Quant-iT PicoGreen dsDNA kit by Thermo Scientific Catalog #: P7589 2Prepare 1XTE buffer from 20X stock solution using nuclease-free water: will need 200 μl/well (for. Picogreen 定量试剂盒兼容大多数微孔板读数仪和荧光计。. Dilute from 20xTE stock (supplied in the PicoGreen kit). The assay is highly selective for double-stranded DNA over RNA, and in the range of 4–1,000 ng, the fluorescence signal is linear with DNA (see “Assay selectivity” on page 1). Quant-iT™ PicoGreen™ dsDNA Assay KitInvitrogen - Thermo FisherCatalog #P11496 Quant-iT™PicoGreen® dsDNA reagent is dissolved in dimethylsulfoxide (DMSO), which freezes below 19 °C. Technical note | Quant-iT and Qubit 1X dsDNA assays the Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. Calibrator DNA content was assessed with Quant-iT™ PicoGreen™ dsDNA Assay Kit (Thermo Fisher Scientific). • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Using the Quant-iTTM PicoGreen® dsDNA reagent and the recommended assay protocol, researchers can quantitate dsDNA in the presence of equi molar concentrations of. 0. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 1 ng/μL to 2000 ng/μL, with an extended upper range to 4000 ng/μL. For a standard. Picogreen assays are compatible with most microplate readers and fluorometers. 50 pg to 2 µg. Product Description. Fluorescence was measured at 485/530 nm and plotted versus the mass of nucleic acid for the DNA alone,. A Qubit dsDNA BR (broad range, 2 to 1000 ng) Assay Kit and Qubit dsDNA HS (high sensitivity, 0. Calibrator DNA content was assessed with Quant-iT™ PicoGreen™ dsDNA Assay Kit (Thermo. Rapidly detect as little as 0. g. Rapidly detect as little as 0. '. no. This protocol is a simplified and condensed version of. quant it picogreen dsdna assay kit reagents. PicoGreen dsDNA quantitation reagents are. Replicates of four were added to a 96-well plate using 2 μL volumes for rows A through D (beige) and 10 μL volumes for rows E through H (blue). • Suitable for use in 96- and 384-well plate formats. Spectral Data: Quant-iT™ PicoGreen® dsDNA reagent 502/523 nm, bound to nucleic acids Quant-iT™ PicoGreen ® dsDNA Reagent and KitsQuant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). 3ng/mL，DNAThe fragment size of the amplicon was assessed using agarose gel electrophoresis and quantified by Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific). Bioz Stars score: 86/100, based on 1 PubMed citations. 25 pg/uL 的 dsDNA。 该定量试剂盒在三个数量级内呈线性，几乎无序列依赖性，可让您准确测定多种来源的 DNA，包括基因组 DNA、病毒 DNA、小提 DNA 或 PCR 扩. 8 List of Tables Molecular Probes amine-reactive dyes—Table 1. The Quant-iT™ dsDNA High-Sensitivity Assay Kit makes DNA quantification easy and accurate. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Picogreen assays are compatible with most microplate readers and fluorometers. The following protocol is intended for the quantification of double-stranded DNA using Quant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). a) 16. Mit den Quant-iT Picogreen dsDNA-Assay-Kits und dsDNA-Reagenzien erzielen Sie präzise und präzise dsDNA-Konzentrationsmessungen über einen breiten dynamischen Bereich. Measure concentration with a high-sensitivity assay (e. The tubes were. io |. Quant-iT ™ 1X dsDNA Assay Kit, High Sensitivity 200 pg−100 ng 1,000 reactions Q33232 Quant-iT ™ 1X dsDNA Assay Kit, Broad-Range 4 ng−2 μg 1,000 reactions Q33267 Quant-iT ™ DNA Assay Kit, High Sensitivity 200 pg−100 ng 1,000 reactions Q33120 Quant-iT ™ DNA Assay Kit, Broad-Range 4 ng−1 μg 1,000 reactions Q33130 Quant-iT ™The Quant-iT™ dsDNA Broad-Range Assay Kit makes DNA quantitation easy and accurate. The Quant-iT PicoGreen dsDNA. Preparing the Reagent On the day of the experiment, prepare an aqueous working solution of the Quant-iT™Quant-iT PicoGreen dsDNA assay for nucleic acid quantification. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. 2mL 1 x 96 wellsAssay Plate with Plate Sealing Foil, D002 *Component can be purchased separately. 710 citations – Kit [P7589] Quant-iT™ PicoGreen™ dsDNA Assay Kit by Thermo Scientific. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. The following protocol is intended for the quantification of double-stranded DNA using Quant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. 50 pg to 2 µg. Figure 2. Rapidly detect as little as 0. We recommend using Quant-IT Picogreen as a high-sensitivity assay to measure DNA concentration in that range . The AccuBlue® High Sensitivity dsDNA Quantitation Kit offers sensitive and selective detection of purified dsDNA samples with a linear detection range from 0. Thermo Fisher quant it picogreen dsdna assay kit. Easy to use UV absorbance alternative assay that can be. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Picogreen assays are compatible with most microplate readers and fluorometers. The DNA standard was Lambda DNA included in the Quant-iT PicoGreen dsDNA Assay Kit (ThermoFisher). Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 04 corresponds to 2 μ g/mL dsDNA solution. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. PicoGreen dsDNA quantitation reagents are. stranded DNA (dsDNA). Prepare the 1x TE buffer by diluting the 20x TE concentrate 1:20 by deionized water. The sample must yield a minimum of 55 ng of genomic DNA to ensure sufficient DNA for quality control (QC) and. The QFX is a powerful solution for all fluorometric applications where enhanced specificity or sensitivity are needed. Quant-iT™ PicoGreen ® dsDNA Reagent (10 vials, each containing 100 µL aliquots) 25 mL of 20X TE buffer; 1 mL of 100 µg/mL Lambda DNA (dsDNA standard) These jellies cheapest cialis uk and pills are can be ingested with or without food. If you would like to use this kit with the Qubit® fluorometer, we have includedconjunction with Molecular Probes' PicoGreen dsDNA Quantitation Reagent enables researchers to quantitate as little as 1 ng/mL of dsDNA. Microplate reader setup for a Quant-iT 1X dsDNA HS Assay. Equal amounts (100 µL) of working PicoGreen quantitation reagent were mixed and incubated for 10 minutes at room temperature, protected from light. Serial dilutions were made to give appropriate cell, seeding numbers. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. A 1:200 dilution of PicoGreen reagent is prepared by adding 10 μl of PicoGreen per 2 mL of 1X TE buffer. The Quant-iT 1X dsDNA BR Assay Kit is accurate for initial DNA sample concentrations of 200 pg/µL to 4000 ng/µL (depending on sample volume. The assay is linear over three orders of magnitude and has little sequence. 125 to 100 ng/mL. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Quant-iT PicoGreen dsDNA Assay Kit a. Within two years of follow-up, the primary endpoint defined as nonfatal myocardial infarction, stroke or transient ischemic attack, cardiovascular death, and >80% target vessel restenosis occurred in 34 patients (43%). Product Type. Picogreen assays are compatible with most microplate readers and fluorometers. Samples ranging in concentration from 0 ng/mL to 1,000 ng/mL were measured in quadruplicate, and the results are shown from the same data plotted in two different ways (Tables 2 and 3). The original sequencing data. The accuracy index is defined in Table 2. 2. The Quant-iTTM PicoGreenTM dsDNA Reagent enables researchers to quantitate as little as 250 pg/mL of dsDNA (50 pg dsDNA in a 200 μL assay volume) with a fluorescence. Learn how to use the Quant-iTTM PicoGreenTM dsDNA Reagent and Kit to quantitate double-stranded DNA (dsDNA) in solution with a fluorometer or a microplate reader. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. 2– 100 ng Quant-iT 1X dsDNA HS Assay Kit – Quant-iT PicoGreen Reagent 1–200 ng Quant-iT OliGreen ssDNA Reagent and Assay Kit. Rapidly detect as little as 0. The PicoGreen is sticking to the sides of the cuvette or assay plate. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. The Qubit dsDNA BR (Broad-Range) Assay Kit, when used with the Qubit Fluorometer, provides an accurate and selective method for the quantitation of DNA samples. 2–400ng/µl (1µl sample in 200µl assay). DNA standards were prepared from concentrated stock herring sperm dsDNA (Sigma, PN-A3294) in TE buffer (tris-EDTA, pH 7. Materials: 1. The following protocol is intended for the quantification of double-stranded DNA using Quant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). Microplate reader setup for a Quant-iT 1X dsDNA HS Assay. The reagent Quant-iT PicoGreen was added to all wells and the samples were incubated for 5 min at room. The kit included 20X TE buffer (200 mM Tris-HCl, 20 mM EDTA, pH 7. 0 fluorometer according to the manufacturer’s protocols; a. For example, 3 µL of the DNA standard mixed with 147 µL of TE is sufficient for the standard curve described in step 2. P7589. PicoGreen dsDNA quantitation reagents are. Samples should be free of RNA contamination and the use of the Qiagen Reaction Cleanup Kit (Qiagen) is recommended. You will need 100 μl diluted PicoGreen reagent per well. Instead of 2 mL, only 1 mL measurement volume was used. このアッセイは、従来のUV吸光度法に比べて感度が何倍も高いため、様々な濃度範囲に対して最適化することが可能です。. 6 Add equivalent volume (100 μl) of diluted PicoGreen to every well (keeping plate in the dark as much as possible). Table 2 summarizes results of the Quant-iT Prepare the first standard Combine 20uL of 100ug/mL lambda DNA from the Quant-IT PicoGreen dsDNA assay kit (Fisher P7589) with 980uL 1X TE to create a 2ug/mL standard. For Research Use. Invitrogen™ Quant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents. Total amount of 1X TE per assay will be 200 μl per well which includes the amount of TE used to dilute the PicoGreen reagent. 10. • Specific for dsDNA in the presence of RNA. 1 mL kit. Compared with the aforementioned methods, the PicoGreen dye binding assay for dsDNA has greater sensitivity, specificity, and linear range. Fluorometric dsDNA Assay. Quantitation using Quant-iT Assay Kits. The Quant-iT family of assay kits—and the related Invitrogen Qubit family of assay kits designed for use with the Invitrogen Qubit 2. P7589. Reagent Search & Data Services Menu Reagent Search. is 1 pg/μL – 5 ng/μL so the sensitivity of this kit can be rated as Ultra High (UHS). 1X dsDNA assay (%) 100 17. 10 x 100 µL. Quant-iT ™ 1X dsDNA Assay Kit, Broad-Range 4 ng−2 μg 1,000 reactions Q33267The supernatant containing the cytosolic extract was either immediately used or stored at − 80 °C until use. Picogreen assays are compatible with most microplate readers and fluorometers. The Quant-iT™ PicoGreen® dsDNA Assay Kit makes for an easy, reliable, and accurate method to quantify dsDNA samples when used in conjunction with the Modulus™ Microplate Fluorometer from Turner BioSystems, Inc. the automated setup to the manual setup of the Quant-iT PicoGreen dsDNA Assay Kit. Data were expressed as mean ± standard deviation (SD) of n = 3 unless otherwise specified. (dsDNA) High Sensitivity (HS) assay, Quant-iT PicoGreen Assay, Bioanalyzer HS DNA assay, and NanoDrop Onec. Preparing the Reagent On the day of the experiment, prepare an aqueous working solution of the Quant-iT™ Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. 3. 2 ng to 100 ng of DNA in the assay tube. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. quant it picogreen dsdna assay kit reagents ( Thermo Fisher ) 86. Mix PicoGreen and DNA by pippetting up and down 5-10 times. 20–1,000 ng Qubit RNA BR Assay Kit 5–100 ng Qubit RNA HS Assay Kit Varioskan LUX Multimode Microplate Reader 2–1,000 ng Quant-iT dsDNA BR Assay Kit 0. 1. Barcoded amplicons were pooled in. The Quant-iT 1X dsDNA HS Assay Kit is accurate for initial sample concentrations ranging from 10 pg/µL to 100 ng/µL, providing a core detection range of 0. quant it picogreen dsdna assay kit , 2023-10. 99 to $1999. Add-and-read format makes measuring low concentrations of dsDNA simple—no dilutions, no extra tubes. RESULTS The OT-2 Constructs Highly Uniform LibrariesThermo Fisher double stranded dna quant it picogreen dsdna assay kit Double Stranded Dna Quant It Picogreen Dsdna Assay Kit, supplied by Thermo Fisher, used in various techniques. Protocols All Picogreen reactions and measurements werethe Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. PicoGreen assay. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Rapidly detect as little as 0. DNA standards ranging from 1000 ng/mL to 50 pg/mL were assayed using the Quant-iT PicoGreen dsDNA Assay and SpectraMax M5 microplate reader. in the assay in order to reduce waste and still achieve comparable results. ZERO BIAS - scores, article reviews, protocol conditions and moreGot it. Natural cures with herbs are. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. 0 fluorometer (Selection Guide for the Qubit and Quant-iT Assay Kits. Tris-EDTA Buffer (10 mM tris, 1 mM EDTA), pH8. Fluorescence was measured at. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Reagents and materials PicoGreen dsDNA Quantitation Kit, Invitrogen Molecular Probes, #P-7589 or equivalent. The Qubit 1X dsDNA BR Standards includes standards at two concentrations, 0 and 100 ng/µL. DNA was quantified by the Quant-it PicoGreen dsDNA Assay Kit (Invitrogen Life technologies, UK) according to the manufacturer’s protocol. Spectrofluorometry was performed based on guidelines from ThermoFisher Scientific Inc. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. The AccuBlue® Broad Range RNA Quantitation Kit is highly selective for RNA over dsDNA. 2 to 100 ng) Assay Kit were used with a Qubit 3. Catalog Number. The Quant-iT dsDNA Broad-Range Kit is designed for assaying samples containing 2–1000 ng of DNA. A12216) Metabolic (glucose) • Amplex Red Glucose/Glucose Oxidase Assay Kit (Cat. P7589. Table 2 summarizes results of the. Q33130) Metabolic (cholesterol) • Amplex Red Cholesterol Assay Kit (Cat. UV흡광 결과보다 민감도 우수. Picogreen assays are compatible with most microplate readers and fluorometers. 2 to 2,000 ng/µL, providing a detection range of 4−2,000 ng. ZERO BIAS . The AccuBlue® High Sensitivity assay is also available without any DNA standards ( 31008 ), for those that wish to use their own standard. Catalog Number. The DeNovix dsDNA Broad Range Assay is ideal for measuring sample concentrations ranging from 0. (Waltham, MA, United States) using the Quant-iT TM PicoGreen TM dsDNA Assay Kit. 2000 ng/mL of lamda dsDNA was serially diluted in 1XTE before the addition of the PicoGreen working solution. Rapidly detect as little as 0. For example, if the assay requires 50 wells, add 600 L of PicoGreen to 14. Picogreen assays are compatible with most microplate readers and fluorometers. Triplicate 10 µL samples of DNA (), Escherichia coli rRNA or a 1:1 mixture of DNA and RNA were assayed with the Quant-iT dsDNA High-Sensitivity Assay Kit (). The superior sensitivity and performance of the Modulus™ Microplate Fluorometer allows for dsDNA detection as. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Selection guide for the Qubit and Quant-iT Assay Kits—Table 8. Highly selective for dsDNA over ssDNA or RNA. 蛍光を用いたDNAの定量法の一つとしてInvitrogen™ Quant-iT™ PicoGreen™ dsDNA Assay Kit が利用されて. PicoGreen dsDNA quantitation reagents are. The method for this assay follows the instructions in the product information sheet for Quant-iT PicoGreen dsDNA Reagent and Kits from Molecular Probes, except that the assay volume is proportionately reduced from 2. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile, distilled, DNase-free water. The Quant-iT™ PicoGreen® dsDNA reagent is a proprietary, asymmetrical cyanine dye. Vortex solution briefly to mix well and centrifuge for 5 sec to bring liquid to bottom of tube; then dispense for use in the assay. Rapidly detect as little as 0. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. 2 to 1000 ng) Assay Kit and Qubit dsDNA HS (high sensitivity, 0. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. ZERO BIAS - scores, article reviews, protocol conditions and moreComparison of dsDNA dose-response using the Helixyte Green™ Fluorimetric dsDNA Quantitation Kit *High Sensitivity* (blue) with Quant-iT™ PicoGreen ® dsDNA Assay Kit (red ). Briefly, 2 µL of sample was diluted in 498 µL TE. The kit contains. Below are some easy steps to perform quantification in plates with Pico488 quantification kit by Lumiprobe (analog of Quant-iT® PicoGreen® dsDNA Assay Kit). When 5–500 pg salmon dsDNA or 3–1000 pg control dsDNA (calf thymus dsDNA standard) is added to AccuClear a linear response is observed (Fig. 2023. The Quantus™ NGS Starter package is cost-effective and easy to incorporate into your laboratory. Specifications Documents Scientific Resources Application Notes Quant-iT PicoGreen dsDNA AssayキットおよびdsDNA試薬を使用すると、幅広いダイナミックレンジでdsDNA濃度測定を正確かつ精密に行うことができます。. A 96 plate well was filled with 100 µl of dye and 100 µl of DNA, a 384 well with 10 µl of each solution. Fluorometer. to each well. Product Type. (2012). DNA standards (λ DNA) and DNA samples were stained using a Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen, Eugene, OR, USA). (The same tips can be used. 2K. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Related applications: Nucleic Acid Quantitation. Price from $9. Quant-iT™ PicoGreen® dsDNA Assay Kit *2,000 assays* (includes PicoGreen® Reagent, 10X buffer, and standard λ DNA) Quant-iT™ RiboGreen® RNA Reagent *2,000 assays* 10 ng/µl to 100 ng/µl RNA (>500 bp; not specific for RNA over DNA) RNase protection assays Microarray samplesIn this research six cyanine fluorophores for the quantification of dsDNA in the pg-ng range, without amplification, are compared under exactly identical conditions: EvaGreen, SYBR Green, PicoGreen, AccuClear, AccuBlue NextGen and YOYO-1. If your are using plastic cuvettes or assay plates, reduce this problem by including 0. Development and characterization of a novel host cell DNA assay using ultra-sensitive fluorescent nucleic acid stain “PicoGreen. (D–G) TLR stimulation was measured using a HEK293-TLR reporter. 2–100 ng: 2000: O11492 O7582: Quant-iT OliGreen ssDNA Assay Kit and Reagent (Ex/Em = 498/518 nm) ssDNA: 1–200 ng: 2000: R11490 R11491: Quant-iT RiboGreen RNA Assay Kit and Reagent (Ex/Em = 500/525 nm) RNA: 20–1000 ng: 2000: Q32883:DNA concentration was quantified with Quant-iT PicoGreen dsDNA Assay kit (Invitrogen) on a Tecan plate reader. Bioz Stars score: 86/100, based on 1 PubMed citations. Facebook. The PicoGreen dsDNA quantitation reagent and kits are ideal for PCR-based assays, microarray samples, DNA damage assays, enzyme activity assays, genomic DNA quantitation, measuring dsDNA in complex mixtures, and viral DNA quantitation. DNA samples with a concentration determined by 260 nm absorbance higher than 100 ng/mL were diluted. In the Quant-iT/PicoGreen assay, DNA and working solution were mixed 1:1. Optimized for fluorescence microplate readers. Dynamic range and sensitivity of the Quant-iTTM PicoGreen® dsDNA assay. • Specific for dsDNA in the presence of RNA. Note: Allow the Quant-iT ™ RiboGreen RNA Reagent to warm to room temperature before. Quant-iT ™ 1X dsDNA Assay Kit, Broad-Range 4 ng−2 μg 1,000 reactions Q33267dsDNA in the presence of equimolar concentrations of ssDNA and RNA with minimal effect on the quantitative results. Rapidly detect as little as 0. assay. Catalog number: P7581. Rapidly detect as little as 0. Quant-iT PicoGreen dsDNA Assay Kit and Reagent (Ex/Em = 502/523 nm) dsDNA: 0. Cloning, expression and proteins purification. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. 0 fluorometer according to the manufacturer’s protocols; a sample volume of 1 μl was. high-range. Perform dsDNA quantification easily and quickly with Quant-iT dsDNA assay kits. 1. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. pDNA encapsulation efficiency of LNP 10 and LNP 20 was measured by using a Quant-iT Pico-Green dsDNA assay kit (ThermoFisher Scientific,. g. Contents and Storage Information. The PicoGreen dsDNA quantitation assay can be used when selectively detecting as little as 25 pg/ml of dsDNA with free nucleotides, RNA and ssDNA present, having minimal impact on the intensity of. Note: The 20X TE buffer (Component B) included in the PicoGreen dsDNA Quantitation Kit is certified to be nucleic acid-free and DNase-free. 99 to $1999. 2. For example, if the amount of the linear dsDNA library available is 20 ng, the linear dsDNA library input should be 10 ng, and the linear dsDNA library concentration should be at least 0. This protocol is a simplified and condensed. High Sensitivity and Broad Range dsDNA Quantitation Superior sensitivity and broad dynamic range A ccuBlue™ dsDNA quantitation kits offer ultra-sensitive and selective detection of dsDNA with minimal effects from common contaminants. VIEWS. No. Quant-iT PicoGreen Reagent Final DNA Concentration in Quant-iT PicoGreen Assay 0 1000 25 ng/mL 900 100 1000 2. 50 pg to 2 µg. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. 2–100 ng, the fluorescence signal is linear with DNA. This reagent is also included with the Quant-iT RiboGreen RNA Assay Kit and comes complete with buffer and RNA. 2009;49(4):997–1002. Rapidly detect as little as 0. 使用 PicoGreen dsDNA 定量试剂盒和试剂时，即使在存在 ssDNA、RNA 和游离核苷酸的情况下，也可快速检测低至 0. The Quant-iT dsDNA High-Sensitivity Assay Kit and the Quant-iT dsDNA Broad-Range Assay Kit make DNA quantification easy and accurate. 99. ru by: 1. Samples were excited at 480 nm and the fluorescence was measured at 520 nm using a TECAN Spark multimode reader. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. Qubit assays are available for double- and single-stranded DNA (dsDNA and ssDNA), RNA, microRNA, and protein. 9 Q33230 9 500 28. Rapidly detect as little as 0. SpectraMax Plus Spectrophotometer (Molecular Devices) and Picogreen quantitation was performed using Quant-iT™ PicoGreen® dsDNA Assay Kit From Molecular Probes (Invitrogen). Picogreen assays are compatible with most microplate readers and fluorometers. Quant-iT PicoGreen dsDNA Assay Kit. The assay is highly selective for double- stranded DNA over RNA, and in the. 5 – 1,000 ng/ mL DNA with a high dynamic range (Fig. 5. Using this data as a starting point, the optimal conditions (e. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. 0 mL to 200 uL to fit a 96-well microplate format. The assay is performed at room temperature, and the signal is stable for 3 hours. • Specific for dsDNA in the presence of RNA. 2 - 100 ng/ml 480/520 NaCl, MgCl 2Quant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen ssDNA Reagent: ssDNA : 200 pg–2 µg : 500/525 : 2,000 : O11492 O7582: Quant-iT RiboGreen RNA Assay Kit Quant-iT. Prepare the rest of the standards , to prepare enough working solution to assay 100 samples in 200 µL volumes, add 50 µL Quant-iT ™ RiboGreen RNA Reagent to 9. The following protocol is intended for the quantification of double-stranded DNA using Quant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). The use of a serial dilution of dsDNA standards whileThe quantity of ChIP DNA should first be determined with the Quant-iT™ PicoGreen dsDNA Reagent Kit (Invitrogen). Description. Optimized for fluorescence microplate readers. No. DNA content was then determined using the Quant-iT PicoGreen dsDNA assay kit per manufacturer’s instructions. The reagent must be completely thawed before using it by bringing it to room temperature. The total amount of DNA expected is in the range of 3–100 ng, with concentrations as low as 0. 5ul of 12uM primer diluted in ClontechPicoGreen is highly linear (R 2 ≥ 0. Quant It Picogreen Dsdna Assay Kit, supplied by Thermo Fisher, used in various techniques. Table 2 summarizes results of the. This protocol is a simplified and condensed version of. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA,. This protocol is a simplified and This protocol is a simplified and condensed version of the full protocol from the manufacturer. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts.